Journal: Biology
Article Title: Neuroprotective, Antioxidant and Anti-Inflammatory Effect of Greek Pomegranate Seed Oil on N2a Neuroblastoma Cells and Mild Cognitive Impairment Patients
doi: 10.3390/biology14050548
Figure Lengend Snippet: The effects of PSO on LPS-induced expression of inflammatory and oxidative stress markers in N2a cells treated with PSO. ( a ) Representative Western blot images showing the expression levels of inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and tumor necrosis factor-alpha (TNF-α). Groups include Control, LPS (1 µg/ mL), LPS co-treatment with different concentrations of PSO (0.2, 10, and 25 µg/mL), and PSO (0.2, 10, and 25 µg/mL) (see ). ( b ) Quantification of total iNOS protein levels normalized to β-actin. ( c ) Quantification of IL-1β protein levels normalized to β-actin. ( d ) Quantification of TNF-α protein levels normalized to β-actin. Statistical significance: * p < 0.05; ** p < 0.01; **** p < 0.0001, with asterisk (*) indicating the comparison versus control group; # p < 0.05, ## p < 0.01, with (#) indicating the comparison with the LPS-treated group. Data are presented as the mean ± SD.
Article Snippet: For the detection of APP, Aβ 42 , tau, p-tau181, iNOS, IL-1β, TNF-α and SOD1, the following primary antibodies were employed: anti-APP mouse monoclonal antibody (#60342-1-Ig, Proteintech, Manchester, UK), anti-Aβ 42 rabbit monoclonal antibody (#14974, Cell Signaling Technology, Danvers, MA, USA), anti-rabbit tau monoclonal antibody (#46687, Cell Signaling Technology, Danvers, MA, USA), anti-p-tau181 rabbit monoclonal antibody (#12885, Cell Signaling Technology, Danvers, MA, USA), anti-iNOS rabbit polyclonal antibody (#18985-1-AP, Proteintech, Manchester, UK), anti-IL-1β rabbit monoclonal antibody (#L0328Y, Cusabio, Houston, TX, USA), anti-TNF-α mouse monoclonal antibody (#sc-52746, Santa Cruz Biotechnology, Heidelberg, Germany), and an anti-SOD1 rabbit polyclonal antibody (#10269-1-AP, Proteintech, Manchester, UK).
Techniques: Expressing, Western Blot, Control, Comparison
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